A Crack in Creation

April 10, 2017
Doudna, professor of biology at UC-Berkeley, and Sternberg, her former graduate student and current collaborator, explain the basics of the potentially revolutionary CRISPR technology, the events leading up to Doudna’s discovery of that technology, and the ethical dilemmas posed by the newfound ability to alter any living being’s genetic composition. The authors describe the biological mechanisms in a way that nonspecialists can appreciate, though the simplistic diagrams scattered throughout add little to the text. They also enthusiastically survey many of the uses to which CRISPR technology has already been applied, noting the great interest by venture capitalists who have already invested well over $1 billion in this technology. Doudna and Sternberg make a clear distinction between manipulating reproductive and non-reproductive cells, since the former can cause permanent evolutionary shifts. The second half of the book delves into the ethical implications arising from this difference, thoughtfully covering effects on both human and non-human species. Though the authors note that science involves both “competition and collaboration,” they avoid discussion of the myriad conflicts that exist in this exciting new field—an absence that makes the rosy picture presented in this otherwise excellent book just a bit too unbelievable. Illus. Agent: Max Brockman, Brockman Inc.

Starred review from April 1, 2017
A pair of biochemists offer a fresh examination of the "newest and arguably most effective genetic-engineering tool."Biological spectaculars--e.g., genetic engineering, cloned sheep, in vitro fertilization--have produced headlines and bestsellers but flopped where it counts: they don't save many lives. CRISPR (clustered regularly interspaced short palindromic repeats) is changing that, write Doudna (Chemistry and Molecular Biology/Univ. of California; co-author: Molecular Biology: Principles and Practice, 2011, etc.) and Sternberg in this enthusiastic and definitely not dumbed-down account of gene manipulation that, unlike earlier methods, is precise and easy. In the first half of the book, "The Tool," the authors summarize a century of research but focus on the discovery, in the early 2000s, that bacteria possess an ingenious immune system that destroys invading viruses by cutting their DNA into pieces. Within the past decade, researchers converted this into an ingenious technique for literally debugging DNA: putting in good genes in the place of bad. "Because CRISPR allows precise and relatively straightforward DNA editing," write the authors, "it has transformed every genetic disease--at least, every disease for which we know the underlying mutation--into a potentially treatable target." The second half, "The Task," describes the miraculous powers of CRISPR to cure disease and control evolution--but not yet. Replacing a single defective gene cures muscular dystrophy in mice; clinical trials in humans for this and similar disorders (sickle-cell, hemophilia, cystic fibrosis) are in the works. CRISPR can't yet cure cancer, prevent AIDS, wipe out malaria, revive the wooly mammoth, or regenerate a limb, but an avalanche of startups (Doudna's included) is betting billions that it eventually will. An important book about a major scientific advance but not for the faint of heart. Readers not up to speed on high school biology should prepare themselves with a good popular primer on DNA, such as Matthew Cobb's Life's Greatest Secret (2015).

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